J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (7): 606-612.DOI: 10.1111/jipb.12305

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Getting started in mapping-by-sequencing

Héctor Candela*, Rubén Casanova-Sáez and José Luis Micol*   

  1. Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, Elche, Spain
  • Received:2014-06-13 Accepted:2014-10-28 Published:2014-10-31
  • About author:Current address: Department of Forest Genetics and Plant Physiology, Umeä Plant Science Center, Swedish University of Agricultural Sciences, Umea SE-901 83, Sweden.
    **Correspondence: E-mail: hcandela@umh.es; jlmicol@umh.es

Abstract:

Next-generation sequencing (NGS) technologies allow the cost-effective sequencing of whole genomes and have expanded the scope of genomics to novel applications, such as the genome-wide characterization of intraspecific polymorphisms and the rapid mapping and identification of point mutations. Next-generation sequencing platforms, such as the Illumina HiSeq2000 platform, are now commercially available at affordable prices and routinely produce an enormous amount of sequence data, but their wide use is often hindered by a lack of knowledge on how to manipulate and process the information produced. In this review, we focus on the strategies that are available to geneticists who wish to incorporate these novel approaches into their research but who are not familiar with the necessary bioinformatic concepts and computational tools. In particular, we comprehensively summarize case studies where the use of NGS technologies has led to the identification of point mutations, a strategy that has been dubbed “mapping-by-sequencing”, and review examples from plants and other model species such as Caenorhabditis elegans, Saccharomyces cerevisiae, and Drosophila melanogaster. As these technologies are becoming cheaper and more powerful, their use is also expanding to allow mutation identification in species with larger genomes, such as many crop plants.

 

Candela H, Casanova-Sáez R, Micol JL (2015) Getting started in mapping-by-sequencing. J Integr Plant Biol 57: 606–612. doi: 10.1111/jipb.12305

Key words: Mapping-by-sequencing, massively parallel sequencing, mutation identification, SHOREmapping, whole-genome re-sequencing

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