Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula

doi:10.1111/jipb.12457

J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (7): 618-622.DOI: 10.1111/jipb.12457

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Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula

Maria Elisa Sabatini¹, Mattia Donà^1†, Paola Leonetti², Andrea Minio³, Massimo Delledonne³, Daniela Carbonera¹, Massimo Confalonieri⁴, Giorgio Giraffa⁴ and Alma Balestrazzi^1*

¹Department of Biology and Biotechnology ‘L. Spallanzani‘ 27100 Pavia, Italy
²Institute for Sustainable Plant Protection, National Research Council (CNR), Bari, Italy
³Department of Biotechnology, University of Verona, Verona, Italy
⁴Fodder and Dairy Production Research Centre, Lodi, Italy

Received:2015-04-20 Accepted:2015-12-18 Published:2015-12-23
About author:^†Present address: Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr Gasse 3, 1030 Vienna, Austria
^*Correspondence: E-mail: alma.balestrazzi@unipv.it

Abstract

Abstract:

The role of plant tyrosyl-DNA phosphodiesterase 1α in genome stability is studied using a Medicago truncatula MtTdp1α-depleted line. Lack of MtTdp1α results in a 39% reduction of methylated cytosines as compared to control. RNA-Seq analyses revealed that 11 DNA transposons and 22 retrotransposons were differentially expressed in the Tdp1α-2a line. Among them all, DNA transposons (MuDR, hAT, DNA3-11_Mad) and seven retrotransposons (LTR (Long Terminal Repeat)/Gipsy, LTR/Copia, LTR and NonLTR/L1) were down-regulated, while the 15 retrotransposons were up-regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.

Key words: DNA methylation, Medicago truncatula, RNA-Seq, transposable elements, tyrosyl-DNA phosphodiesterase

Maria Elisa Sabatini, Mattia Donà, Paola Leonetti, Andrea Minio, Massimo Delledonne, Daniela Carbonera, Massimo Confalonieri, Giorgio Giraffa, and Alma Balestrazzi. Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula[J]. J Integr Plant Biol., 2016, 58(7): 618-622.

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Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula

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