J Integr Plant Biol ›› 2017, Vol. 59 ›› Issue (1): 2-14.DOI: 10.1111/jipb.12504

• Cell and Developmental Biology •     Next Articles

SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean

Bin Cai1, Xiangxiong Kong1, Chao Zhong2, Suli Sun2, Xiao Feng Zhou3, Yin Hua Jin1, Youning Wang4, Xia Li4, Zhendong Zhu2 and Jing Bo Jin1*   

  1. 1Key Laboratory of Plant Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China
    2National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3Department of Ornamental Horticulture, China Agricultural University, Beijing 100193, China
    4State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2016-08-20 Accepted:2016-10-18 Published:2016-10-20
  • About author:*Correspondence: E-mail: Jing Bo Jin (jinjb@ibcas.ac.cn)

Abstract:

SIZ1 is a small ubiquitin-related modifier (SUMO) E3 ligase that mediates post-translational SUMO modification of target proteins and thereby regulates developmental processes and hormonal and environmental stress responses in Arabidopsis. However, the role of SUMO E3 ligases in crop plants is largely unknown. Here, we identified and characterized two Glycine max (soybean) SUMO E3 ligases, GmSIZ1a and GmSIZ1b. Expression of GmSIZ1a and GmSIZ1b was induced in response to salicylic acid (SA), heat, and dehydration treatment, but not in response to cold, abscisic acid (ABA), and NaCl treatment. Although GmSIZ1a was expressed at higher levels than GmSIZ1b, both genes encoded proteins with SUMO E3 ligase activity in vivo. Heterologous expression of GmSIZ1a or GmSIZ1b rescued the mutant phenotype of Arabidopsis siz1-2, including dwarfism, constitutively activated expression of pathogen-related genes, and ABA-sensitive seed germination. Simultaneous downregulation of GmSIZ1a and GmSIZ1b (GmSIZ1a/b) using RNA interference (RNAi)-mediated gene silencing decreased heat shock-induced SUMO conjugation in soybean. Moreover, GmSIZ1RNAi plants exhibited reduced plant height and leaf size. However, unlike Arabidopsis siz1-2 mutant plants, flowering time and SA levels were not significantly altered in GmSIZ1RNAi plants. Taken together, our results indicate that GmSIZ1a and GmSIZ1b mediate SUMO modification and positively regulate vegetative growth in soybean.

SUMOylation is a rapid and reversible post-translational process, which affects protein-protein interactions, protein targeting, enzymatic activity and protein stability. However, the role of SUMOylation in soybean is unknown. Here, we report that two soybean SUMO E3 ligases, GmSIZ1a and GmSIZ1b, mediate SUMO modifications and positively regulate vegetative growth in soybean.

Key words: SIZ1, soybean, SUMO, SUMO E3 ligase, vegetative growth

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