J Integr Plant Biol. ›› 2017, Vol. 59 ›› Issue (7): 475-495.DOI: 10.1111/jipb.12549

• Metabolism and Biochemistry • Previous Articles     Next Articles

Phenolic metabolism and molecular mass distribution of polysaccharides in cellulose-deficient maize cells

María de Castro1,2, Romina Martínez-Rubio1, José L. Acebes1, Antonio Encina1, Stephen C. Fry2 and Penélope García-Angulo1*   

  1. 1Área de Fisiología Vegetal. Dpto. Ingeniería y Ciencias Agrarias. Facultad de Biología y Ciencias Ambientales, Universidad de León, Leon E-24071, Spain
    2The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, School of Biological Sciences, Edinburgh EH9 3BF, UK
  • Received:2017-03-30 Accepted:2017-04-21 Published:2017-05-05
  • About author:*Correspondences: E-mail: Penélope García-Angulo (pgara@unileon.es)


As a consequence of the habituation to low levels of dichlobenil (DCB), cultured maize cells presented an altered hemicellulose cell fate with a lower proportion of strongly wall-bound hemicelluloses and an increase in soluble extracellular polymers released into the culture medium. The aim of this study was to investigate the relative molecular mass distributions of polysaccharides as well as phenolic metabolism in cells habituated to low levels of DCB (1.5 μM). Generally, cell wall bound hemicelluloses and sloughed polymers from habituated cells were more homogeneously sized and had a lower weight-average relative molecular mass. In addition, polysaccharides underwent massive cross-linking after being secreted into the cell wall, but this cross-linking was less pronounced in habituated cells than in non-habituated ones. However, when relativized, ferulic acid and p-coumaric acid contents were higher in this habituated cell line. Feasibly, cells habituated to low levels of DCB synthesized molecules with a lower weight-average relative molecular mass, although cross-linked, as a part of their strategy to compensate for the lack of cellulose.

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