J Integr Plant Biol. ›› 2017, Vol. 59 ›› Issue (11): 810-824.DOI: 10.1111/jipb.12570

Special Issue: Plant Signal Transduction

• Plant Reproduction Biology • Previous Articles     Next Articles

Arabidopsis CPR5 regulates ethylene signaling via molecular association with the ETR1 receptor

Feifei Wang1†, Lijuan Wang1†, Longfei Qiao1†, Jiacai Chen1, Maria Belen Pappa2, Haixia Pei1, Tao Zhang1, Caren Chang2 and Chun-Hai Dong1*   

  1. 1College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China
    2Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
  • Received:2017-04-10 Accepted:2017-07-11 Published:2017-11-13
  • About author:These authors contributed equally to this work
    *Correspondence: E-mail: Chun-Hai Dong (chunhai79@yahoo.com)

Abstract:

The plant hormone ethylene plays various functions in plant growth, development and response to environmental stress. Ethylene is perceived by membrane-bound ethylene receptors, and among the homologous receptors in Arabidopsis, the ETR1 ethylene receptor plays a major role. The present study provides evidence demonstrating that Arabidopsis CPR5 functions as a novel ETR1 receptor-interacting protein in regulating ethylene response and signaling. Yeast split ubiquitin assays and bi-fluorescence complementation studies in plant cells indicated that CPR5 directly interacts with the ETR1 receptor. Genetic analyses indicated that mutant alleles of cpr5 can suppress ethylene insensitivity in both etr1-1 and etr1-2, but not in other dominant ethylene receptor mutants. Overexpression of Arabidopsis CPR5 either in transgenic Arabidopsis plants, or ectopically in tobacco, significantly enhanced ethylene sensitivity. These findings indicate that CPR5 plays a critical role in regulating ethylene signaling. CPR5 is localized to endomembrane structures and the nucleus, and is involved in various regulatory pathways, including pathogenesis, leaf senescence, and spontaneous cell death. This study provides evidence for a novel regulatory function played by CPR5 in the ethylene receptor signaling pathway in Arabidopsis.

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