J Integr Plant Biol. ›› 2017, Vol. 59 ›› Issue (12): 866-880.DOI: 10.1111/jipb.12597

• Functional Omics and Systems Biology • Previous Articles     Next Articles

Genetic dissection of top three leaf traits in rice using progenies from a japonica × indica cross

Changbin Yin1, Huihui Li1, Zhigang Zhao2, Zhiquan Wang2,3, Shijia Liu2, Liangming Chen2, Xi Liu2, Yunlu Tian2, Juan Ma1, Lidong Xu2, Dashuang Zhang4, Susong Zhu4, Danting Li5, Jianmin Wan1 and Jiankang Wang1*   

  1. 1The National Key Facility for Crop Gene Resources and Genetic Improvement, and Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2National Key Laboratory for Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China
    3Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
    4Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
    5Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
  • Received:2017-06-12 Accepted:2017-09-04 Published:2017-09-06
  • About author:*Correspondences: E-mail: Jiankang Wang (wangjiankang@caas.cn)


The size of the top three leaves of rice plants is strongly associated with yield; thus, it is important to consider quantitative traits representing leaf size (e.g., length and width) when breeding novel rice varieties. It is challenging to measure such traits on a large scale in the field, and little is known about the genetic factors that determine the size of the top three leaves. In the present study, a population of recombinant inbred lines (RILs) and reciprocal single chromosomal segment substitution lines (SSSLs) derived from the progeny of a japonica Asominori × indica IR24 cross were grown under four diverse environmental conditions. Six morphological traits associated with leaf size were measured, namely length and flag leaf, length and flag, second and third leaves. In the RIL population, 49 QTLs were identified that clustered in 30 genomic region. Twenty-three of these QTLs were confirmed in the SSSL population. A comparison with previously reported genes/QTLs revealed eight novel genomic regions that contained uncharacterized ORFs associated with leaf size. The QTLs identified in this study can be used for marker-assisted breeding and for fine mapping of novel genetic elements controlling leaf size in rice.

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