J Integr Plant Biol. ›› 2018, Vol. 60 ›› Issue (2): 160-172.DOI: 10.1111/jipb.12613

Special Issue: Rice Genomics and Agriculture

• Research Articles • Previous Articles     Next Articles

A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway

Xiao-Bo Zhang, Bao-Hua Feng, Hui-Mei Wang, Xia Xu, Yong-Feng Shi, Yan He, Zheng Chen, Atul Prakash Sathe, Lei Shi and Jian-Li Wu*   

  1. State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China
  • Received:2017-08-13 Accepted:2017-11-24 Published:2018-02-13
  • About author:These authors contributed equally to this work
    *Correspondence: E-mail: Jian-Li Wu (beishangd@163.com)

Abstract:

We previously reported a spotted-leaf mutant pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae. Here, we report the map-based cloning of the causal gene OsPELOTA (originally termed splHM47). We identified a single base substitution from T to A at position 556 in the coding sequence of OsPELOTA, effectively mutating phenylalanine to isoleucine at position 186 in the translated protein sequence. Both functional complementation and over-expression could rescue the spotted-leaf phenotype. OsPELOTA, a paralogue to eukaryotic release factor 1 (eRF1), shows high sequence similarity to Drosophila Pelota and also localizes to the endoplasmic reticulum and plasma membrane. OsPELOTA is constitutively expressed in roots, leaves, sheaths, stems, and panicles. Elevated levels of salicylic acid and decreased level of jasmonate were detected in the pelota mutant. RNA-seq analysis confirmed that genes responding to salicylic acid were upregulated in the mutant. Our results indicate that the rice PELOTA protein is involved in bacterial leaf blight resistance by activating the salicylic acid metabolic pathway.

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