J Integr Plant Biol. ›› 2018, Vol. 60 ›› Issue (2): 112-129.DOI: 10.1111/jipb.12621

• Research Articles • Previous Articles     Next Articles

TSC1 enables plastid development under dark conditions, contributing to rice adaptation to transplantation shock

Xiaoliang Shi1†, Sunlu Chen1†, Yu Peng1, Yufeng Wang1, Jiugeng Chen2, Zhanghua Hu3, Baohe Wang4, Aihong Li4, Daiyin Chao2, Yuhong Li4* and Sheng Teng1*   

  1. 1Laboratory of Photosynthesis and Environmental Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Science, Shanghai 200032, China
    2National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
    3Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
    4Rice Breeding Center, Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou 225007, China
  • Received:2017-11-06 Accepted:2017-12-04 Published:2018-02-13
  • About author:These authors contributed equally to this work
    **Correspondence: Email: Sheng Teng (steng@sibs.ac.cn, Dr. Teng is fully responsible for the distribution of all materials associated with this article); Yuhong Li (yhlirice@163.com)


Since its domestication from wild rice thousands of years ago, rice has been cultivated largely through transplantation. During transplantation from the nursery to the paddy field, rice seedlings experience transplantation shock which affects their physiology and production. However, the mechanisms underlying transplantation shock and rice adaptation to this shock are largely unknown. Here, we isolated a transplant-sensitive chloroplast-deficient (tsc1) rice mutant that produces albino leaves after transplantation. Blocking light from reaching the juvenile leaves and leaf primordia caused chloroplast deficiencies in transplanted tsc1 seedlings. TSC1 encodes a noncanonical adenosine triphosphate-binding cassette (ABC) transporter homologous to AtNAP14 and is of cyanobacterial origin. We demonstrate that TSC1 controls plastid development in rice under dark conditions, and functions independently of light signaling. However, light rescued the tsc1 mutant phenotype in a spectrum-independent manner. TSC1 was upregulated following transplantation, and modulated the iron and copper levels, thereby regulating prolamellar body formation during the early P4 stage of leaf development. Therefore, TSC1 is indispensable for plastid development in the absence of light, and contributes to adaptation to transplantation shock. Our study provides insight into the regulation of plastid development and establishes a framework for improving recovery from transplantation shock in rice.

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