J Integr Plant Biol ›› 2018, Vol. 60 ›› Issue (7): 541-561.DOI: 10.1111/jipb.12648

• Cell and Developmental Biology • Previous Articles     Next Articles

Plant G proteins interact with endoplasmic reticulum luminal protein receptors to regulate endoplasmic reticulum retrieval

Shanshan Wang1†, Ke Xie2†, Guoyong Xu3†, Huarui Zhou1, Qiang Guo1, Jingyi Wu1, Zengwei Liao1, Na Liu1, Yan Wang1 and Yule Liu1*   

  1. 1MOE Key Laboratory of Bioinformatics, Center for Plant Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China
    2Advanced Biotechnology and Application Research Center, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China
    3The Institute for Advanced Studies (IAS), Wuhan University, Wuhan 430072, China
  • Received:2018-03-10 Accepted:2018-03-12 Published:2018-07-01
  • About author:There authors contributed equally to this work.
    *Correspondence: Email: Yule Liu (yuleliu@mail.tsinghua.edu.cn)

Abstract:

Maintaining endoplasmic reticulum (ER) homeostasis is essential for the production of biomolecules. ER retrieval, i.e., the retrograde transport of compounds from the Golgi to the ER, is one of the pathways that ensures ER homeostasis. However, the mechanisms underlying the regulation of ER retrieval in plants remain largely unknown. Plant ERD2‐like proteins (ERD2s) were recently suggested to function as ER luminal protein receptors that mediate ER retrieval. Here, we demonstrate that heterotrimeric G protein signaling is involved in ERD2‐mediated ER retrieval. We show that ERD2s interact with the heterotrimeric G protein Gα and Gγ subunits at the Golgi. Silencing of , , or increased the retention of ER luminal proteins. Furthermore, overexpression of Gα, Gβ, or Gγ caused ER luminal proteins to escape from the ER, as did the co‐silencing of ERD2a and ERD2b. These results suggest that G proteins interact with ER luminal protein receptors to regulate ER retrieval.

G proteins have multiple roles inside the cells. In this study, we found a new function of heterotrimeric G proteins. We demonstrated that G protein subunits negatively regulate the ER retrieval by interacting with ER luminal protein receptors, which is responsible for ER retrieval from Golgi to ER.
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