J Integr Plant Biol. ›› 2018, Vol. 60 ›› Issue (9): 864-877.DOI: 10.1111/jipb.12666

Special Issue: Special Issue on Cell Signaling

• Research Articles • Previous Articles     Next Articles

Ethylene-induced microtubule reorientation is essential for fast inhibition of root elongation in Arabidopsis

Yichuan Wang1*, Yusi Ji1, Ying Fu2 and Hongwei Guo1*   

  1. 1Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.
    2State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100193, China

    **
    Correspondences:
    Email: Yichuan Wang (wangyc@sustc.edu.cn); Hongwei Guo (guohw@sustc.edu.cn); Drs. Wang and Guo are fully responsible for the distribution of all materials associated with this article)
  • Received:2018-02-08 Accepted:2018-05-09 Online:2018-05-12 Published:2018-09-01

Abstract:

Microtubule reorientation is a long-standing observation that has been implicated in regulating the inhibitory effect of ethylene on axial elongation of plant cells. However, the signaling mechanism underlying ethylene-induced microtubule reorientation has remained elusive. Here, we reveal, by live confocal imaging and kinetic root elongation assays, that the time courses of ethylene-induced microtubule reorientation and root elongation inhibition are highly correlated, and that microtubule reorientation is required for the full responsiveness of root elongation to ethylene treatment. Our genetic analysis demonstrated that the effect of ethylene on microtubule orientation and root elongation is mainly transduced through the canonical linear ethylene signaling pathway. By using pharmacological and genetic analyses, we demonstrate further that the TIR1/AFBs-Aux/IAAs-ARFs auxin signaling pathway, but not the ABP1-ROP6-RIC1 auxin signaling branch, is essential for ethylene-induced microtubule reorientation and root elongation inhibition. Together, these findings offer evidence for the functional significance and elucidate the signaling mechanism for ethylene-induced microtubule reorientation in fast root elongation inhibition in Arabidopsis.

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