J Integr Plant Biol. ›› 2019, Vol. 61 ›› Issue (11): 1151-1170.DOI: 10.1111/jipb.12749

• Cell and Developmental Biology • Previous Articles     Next Articles

TCP7 functions redundantly with several Class I TCPs and regulates endoreplication in Arabidopsis

Guofang Zhang1,2,3,4†, Hongtao Zhao1,2,3,4*†, Chunguang Zhang1,4†, Xiaoyun Li1,2,3,4, Yuanyuan Lyu1,2,3,4, Dongmei Qi1,2,3,4, Yanwei Cui1,4‡, Lin Hu1,4§, Zhenjie Wang1,4¶, Zheng Liang1,4 and Sujuan Cui1,2,3,4,*   

  1. 1Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang 050024, China
    2Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Hebei Normal University, Shijiazhuang 050024, China
    3Hebei Collaboration Innovation Center for Cell Signaling, Hebei Normal University, Shijiazhuang 050024, China
    4College of Life Science, Hebei Normal University, Shijiazhuang 050024, China

    These authors contributed equally to this work.
    Present address: Sports Science Department, Hebei Sport University, Hebei 050041, China
    §Present address: Renshou NO.1 High School of Sichuan Province, Sichuan 620500, China
    Present address: Beijing Front-tech Information Technology Co. Ltd, Beijing 100085, China
    Email: Hongtao Zhao (zhaohongtao@mail.hebtu.edu.cn); Sujuan Cui (cuisujuan@mail.hebtu.edu.cn. Dr. Cui is fully responsible for the distribution of all materials associated with this article.)
  • Received:2018-10-12 Accepted:2018-11-12 Online:2018-11-26 Published:2019-11-01


TCP (TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR) proteins, a family of plant‐specific transcription factors, play important roles in many developmental processes. However, genetic and functional redundancy among class I TCP limits the analysis of their biological roles. Here, we identified a dominant‐negative mutant of Arabidopsis thaliana TCP7 named leaf curling‐upward (lcu), which exhibits smaller leaf cells and shorter hypocotyls than the wild type, due to defective endoreplication. A septuple loss‐of‐function mutant of TCP7, TCP8, TCP14, TCP15, TCP21, TCP22, and TCP23 displayed similar developmental defects to those of lcu. Genome‐wide RNA‐sequencing showed that lcu and the septuple mutant share many misexpressed genes. Intriguingly, TCP7 directly targets the CYCLIN D1;1 (CYCD1;1) locus and activates its transcription. We determined that the C‐terminus of TCP7 accounts for its transcriptional activation activity. Furthermore, the mutant protein LCU exhibited reduced transcriptional activation activity due to the introduction of an EAR‐like repressive domain at its C‐terminus. Together, these observations indicate that TCP7 plays important roles during leaf and hypocotyl development, redundantly, with at least six class I TCPs, and regulates the expression of CYCD1;1 to affect endoreplication in Arabidopsis.

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