J Integr Plant Biol ›› 2020, Vol. 62 ›› Issue (5): 652-667.DOI: 10.1111/jipb.12822

• Plant-abiotic Interactions • Previous Articles     Next Articles

Photoexcited phytochrome B interacts with brassinazole resistant 1 to repress brassinosteroid signaling in Arabidopsis

Huixue Dong, Jie Liu, Guanhua He1, Pan Liu, Jiaqiang Sun*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing 100081, China

    These authors contributed equally to this work.
    *Correspondence:
    Email: Jiaqiang Sun (sunjiaqiang@caas.cn
  • Received:2018-12-06 Accepted:2019-04-28 Online:2019-05-13 Published:2020-05-01

Abstract:

Photoreceptor phytochrome B (phyB) mediates a variety of light responses in plants. To further elucidate the molecular mechanisms of phyB‐regulated hypocotyl elongation, we performed firefly luciferase complementation imaging (LCI) screening for phyB‐interacting transcription factors (TFs). LCI assays showed that phyB possibly interacts with brassinazoleresistant 1 (BZR1), BZR2, AUXIN RESPONSE FACTOR 6 (ARF6), and several WRKY DNA‐binding TFs in a red light‐dependent manner. Furthermore, biochemical assays demonstrated that photoexcited phyB specifically interacts with non‐phosphorylated BZR1, the physiologically active form of a master TF in brassinosteroid (BR) signaling, and this interaction can be competitively interfered by phytochrome‐interacting factor 4. Furthermore, we showed that phyB can directly interact with the DNA‐binding domain of BZR1 and affect the enrichment of BZR1 on the chromatin of target genes. Moreover, our genetic evidence and RNA‐seq analysis demonstrated that phyB negatively regulates BR signaling. Together, we revealed that photoexcited phyB directly interacts with the TF BZR1 to repress BR signaling in Arabidopsis.

Light signals and endogenous hormone brassinosteroids (BRs) are two antagonistic factors in coordinating hypocotyl cell elongation. Here we revealed that photoexcited phyB negatively regulates BR signaling in Arabidopsis through directly interacting with dephosphorylated BZR1, the physiologically active form of a master transcription factor in BR signaling.
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22