J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (8): 1132-1158.DOI: 10.1111/jipb.12894

Special Issue: Temperature signaling

• Plant-abiotic Interactions • Previous Articles     Next Articles

Proteomic and metabolomic profiling underlines the stage- and time-dependent effects of high temperature on grape berry metabolism

David Lecourieux1*, Christian Kappel2, Stéphane Claverol3, Philippe Pieri1, Regina Feil4, John E. Lunn4, Marc Bonneu3, Lijun Wang5, Eric Gomès1, Serge Delrot1 and Fatma Lecourieux6   

  1. 1UMR1287 EGFV, INRAE, Bordeaux Sciences Agro, Bordeaux University, ISVV, 33140 Villenave d'Ornon, France
    2Institut of Biochemistry and Biology, Potsdam University, D‐14476 Potsdam, Germany
    3Proteome Platform, Bordeaux Functional Genomic Center, Bordeaux University, 33076 Bordeaux, France
    4Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam‐Golm, Germany
    5Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
    6UMR1287 EGFV, CNRS, INRAE, Bordeaux Sciences Agro, Bordeaux University, ISVV, 33140 Villenave d'Ornon, France

    *Correspondence: David Lecourieux(david.lecourieux@inra.fr)
  • Received:2019-10-10 Accepted:2019-12-05 Online:2019-12-12 Published:2020-08-01


Climate change scenarios predict an increase in mean air temperatures and in the frequency, intensity, and length of extreme temperature events in many wine‐growing regions worldwide. Because elevated temperature has detrimental effects on berry growth and composition, it threatens the economic and environmental sustainability of wine production. Using Cabernet Sauvignon fruit‐bearing cuttings, we investigated the effects of high temperature (HT) on grapevine berries through a label‐free shotgun proteomic analysis coupled to a complementary metabolomic study. Among the 2,279 proteins identified, 592 differentially abundant proteins were found in berries exposed to HT. The gene ontology categories “stress,” “protein,” “secondary metabolism,” and “cell wall” were predominantly altered under HT. High temperatures strongly impaired carbohydrate and energy metabolism, and the effects depended on the stage of development and duration of treatment. Transcript amounts correlated poorly with protein expression levels in HT berries, highlighting the value of proteomic studies in the context of heat stress. Furthermore, this work reveals that HT alters key proteins driving berry development and ripening. Finally, we provide a list of differentially abundant proteins that can be considered as potential markers for developing or selecting grape varieties that are better adapted to warmer climates or extreme heat waves.

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