J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (9): 1418-1432.DOI: 10.1111/jipb.12907

• Photosynthesis and Crop Physiology • Previous Articles     Next Articles

Plastid ribosomal protein LPE2 is involved in photosynthesis and the response to C/N balance in Arabidopsis thaliana

Xiaoxiao Dong1†, Sujuan Duan1, 2†, Hong-Bin Wang1 and Hong-Lei Jin2*   

  1. 1School of Life Sciences, Sun Yat‐sen University, Guangzhou 510275, China
    2School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China

    These authors contributed equally to this work.
    *Correspondence:
    Email: Hong‐Lei Jin (jinhl@gzucm.edu.cn)
  • Received:2019-12-01 Accepted:2020-01-09 Online:2020-01-15 Published:2020-09-01

Abstract:

The balance between cellular carbon (C) and nitrogen (N) must be tightly coordinated to sustain optimal growth and development in plants. In chloroplasts, photosynthesis converts inorganic C to organic C, which is important for maintenance of C content in plant cells. However, little is known about the role of chloroplasts in C/N balance. Here, we identified a nuclear‐encoded protein LOW PHOTOSYNTHETIC EFFICIENCY2 (LPE2) that it is required for photosynthesis and C/N balance in Arabidopsis. LPE2 is specifically localized in the chloroplast. Both loss‐of‐function mutants, lpe2‐1 and lpe2‐2, showed lower photosynthetic activity, characterized by slower electron transport and lower PSII quantum yield than the wild type. Notably, LPE2 is predicted to encode the plastid ribosomal protein S21 (RPS21). Deficiency of LPE2 significantly perturbed the thylakoid membrane composition and plastid protein accumulation, although the transcription of plastid genes is not affected obviously. More interestingly, transcriptome analysis indicated that the loss of LPE2 altered the expression of C and N response related genes in nucleus, which is confirmed by quantitative real‐time‐polymerase chain reaction. Moreover, deficiency of LPE2 suppressed the response of C/N balance in physiological level. Taken together, our findings suggest that LPE2 plays dual roles in photosynthesis and the response to C/N balance.

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