J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (9): 1352-1371.DOI: 10.1111/jipb.12910

• Research Articles • Previous Articles     Next Articles

Phototropin- and photosynthesis-dependent mitochondrial positioning in Arabidopsis thaliana mesophyll cells

Md Sayeedul Islam1*, Toan Van Nguyen1,2, Wataru Sakamoto3 and Shingo Takagi1   

  1. 1Department of Biological Sciences, Graduate School of Science, Osaka University, Machikaneyama‐cho 1‐1, Toyonaka, Osaka 560‐0043, Japan
    2Agricultural Genetics Institute, National Key Laboratory for Plant Cell Biotechnology, Pham Van Dong road, Bac Tu Liem district, Ha Noi, Vietnam
    3Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710‐0046, Japan

    *Correspondence:
    Email: Md Sayeedul Islam(islam@bio.sci.osaka-u.ac.jp)
  • Received:2019-07-04 Accepted:2020-01-07 Online:2020-01-21 Published:2020-09-01

Abstract:

Mitochondria are frequently observed in the vicinity of chloroplasts in photosynthesizing cells, and this association is considered necessary for their metabolic interactions. We previously reported that, in leaf palisade cells of Arabidopsis thaliana, mitochondria exhibit blue‐light‐dependent redistribution together with chloroplasts, which conduct accumulation and avoidance responses under the control of blue‐light receptor phototropins. In this study, precise motility analyses by fluorescent microscopy revealed that the individual mitochondria in palisade cells, labeled with green fluorescent protein, exhibit typical stop‐and‐go movement. When exposed to blue light, the velocity of moving mitochondria increased in 30 min, whereas after 4 h, the frequency of stoppage of mitochondrial movement markedly increased. Using different mutant plants, we concluded that the presence of both phototropin1 and phototropin2 is necessary for the early acceleration of mitochondrial movement. On the contrary, the late enhancement of stoppage of mitochondrial movement occurs only in the presence of phototropin2 and only when intact photosynthesis takes place. A plasma‐membrane ghost assay suggested that the stopped mitochondria are firmly adhered to chloroplasts. These results indicate that the physical interaction between mitochondria and chloroplasts is cooperatively mediated by phototropin2‐ and photosynthesis‐dependent signals. The present study might add novel regulatory mechanism for light‐dependent plant organelle interactions.

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