J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (4): 628-633.DOI: 10.1111/jipb.13018

Special Issue: Biotechnology

• New Technologies • Previous Articles     Next Articles

Developing a new model system for potato genetics by androgenesis

Miru Du1†, Ting Wang1†, Qun Lian2, Xiaojie Zhang1, Guohui Xin1, Yuanyuan Pu1, Glenn J. Bryan3 and Jianjian Qi1*   

  1. 1Inner Mongolia Potato Engineering and Technology Research Center, Inner Mongolia University, Hohhot 010021, China
    2Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China
    3Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK

    These authors contributed equally to this work.
    *Correspondence: Jianjian Qi (qijj@imu.edu.cn)
  • Received:2020-07-27 Accepted:2020-09-21 Online:2020-09-23 Published:2021-04-01

Abstract: High heterozygosity and tetrasomic inheritance complicate studies of asexually propagated polyploids, such as potato. Reverse genetics approaches, especially mutant library construction, can be an ideal choice if a proper mutagenesis genotype is available. Here, we aimed to generate a model system for potato research using anther cultures of Solanum verrucosum, a self‐compatible diploid potato with strong late blight resistance. Six of the 23 regenerants obtained (SVA4, SVA7, SVA22, SVA23, SVA32, and SVA33) were diploids, and their homozygosity was estimated to be >99.99% with 22 polymorphic InDel makers. Two lines—SVA4 and SVA32—had reduced stature (plant height ≤80 cm), high seed yield (>1,000 seeds/plant), and good tuber set (>30 tubers/plant). We further confirmed the full homozygosity of SVA4 and SVA32 using whole‐genome resequencing. These two regenerants possess all the characteristics of a model plant: diploidy, 100% homozygosity, self‐compatibility, and amenability to transgenesis. Thus, we have successfully generated two lines, SVA4 and SVA32, which can potentially be used for mutagenesis and as model plants to rejuvenate current methods of conducting potato research.

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