J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (9): 1600-1605.DOI: 10.1111/jipb.13146

Special Issue: Biotechnology

• Breakthrough Reports • Previous Articles     Next Articles

Development of an efficient plant dual cytosine and adenine editor

Rongfang Xu1†, Fanna Kong1†, Ruiying Qin1, Juan Li1, Xiaoshuang Liu1,2 and Pengcheng Wei1,2*   

  1. Key Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
    2 College of Agronomy, Anhui Agricultural University, Hefei 230036, China

    These authors contributed equally to this paper.
    *Correspondence: Pengcheng Wei (weipengcheng@gmail.com)
  • Received:2021-05-18 Accepted:2021-06-27 Online:2021-06-30 Published:2021-09-01

Abstract: An enhanced CDA-like (eCDAL) was established from Japanese lamprey CDA1-like 4 to achieve a high editing frequency in a broad region as a C-terminal cytosine base editors (CT-CBE). Then, a novel plant dual-base editor version 1(pDuBE1) was developed by integrating TadA-8e into eCDAL. The editing efficiency of pDuBE1 could reach to 87.6%, with frequencies of concurrent A-to-G and C-to-T conversions as high as 49.7% in stably transformed plant cells. Our results showed that pDuBE1 could mediate robust dual editing in plant genome, providing a powerful manipulation tool for precise crop breeding and screening platforms for in planta direct evolution.

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