J Integr Plant Biol. ›› 2022, Vol. 64 ›› Issue (3): 688-701.DOI: 10.1111/jipb.13220

• Functional Omics and Systems Biology • Previous Articles     Next Articles

Assembly of yield heterosis of an elite rice hybrid is promising by manipulating dominant quantitative trait loci

Guojing Shen1†, Wei Hu1†, Xianmeng Wang1,2†, Xiangchun Zhou1,2, Zhongming Han1, Ahmed Sherif1,2, Mohammed Ayaad1,3 and Yongzhong Xing1,2*   

  1. 1 National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China
    2 Hubei Hongshan Laboratory, Wuhan 430070, China
    3 Plant Research Department, Nuclear Research Center, Atomic Energy Authority, Abo‐Zaabal 13759, Egypt

    These authors contributed equally to this work.
    *Correspondence: Yongzhong Xing (yzxing@mail.hzau.edu.cn)
  • Received:2021-09-23 Accepted:2022-01-04 Online:2022-01-07 Published:2022-03-01


In the past, rice hybrids with strong heterosis have been obtained empirically, by developing and testing thousands of combinations. Here, we aimed to determine whether heterosis of an elite hybrid could be achieved by manipulating major quantitative trait loci. We used 202 chromosome segment substitution lines from the elite hybrid Shanyou 63 to evaluate single segment heterosis (SSH) of yield per plant and identify heterotic loci. All nine detected heterotic loci acted in a dominant fashion, and no SSH exhibited overdominance. Functional alleles of key yield-related genes Ghd7, Ghd7.1, Hd1, and GS3 were dispersed in both parents. No functional alleles of three investigated genes were expressed at higher levels in the hybrids than in the more desirable parents. A hybrid pyramiding eight heterotic loci in the female parent Zhenshan 97 background had a comparable yield to Shanyou 63 and much higher yield than Zhenshan 97. Five hybrids pyramiding eight or nine heterotic loci in the combined parental genome background showed similar yield performance to that of Shanyou 63. These results suggest that dominance underlying functional complementation is an important contributor to yield heterosis and that heterosis assembly might be successfully promised by manipulating several major dominant heterotic loci.

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