J Integr Plant Biol. ›› 2022, Vol. 64 ›› Issue (10): 1856-1859.DOI: 10.1111/jipb.13342

• Brief Communications • Previous Articles     Next Articles

Cas12a-based on-site, rapid detection of genetically modified crops

Zhiqiang Duan1†, Xiaoliang Yang2†, Xingkun Ji1, Ying Chen3, Xiaomu Niu3, Anping Guo2, Jian‐Kang Zhu4,5,6, Feng Li3*, Zhaobo Lang7* and Hui Zhao2*   

  1. 1 Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off‐Season Reproduction Regions, Institute of Tropical Bioscience and Biotechnology & San Ya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
    2 Bellagen Biotechnology Co. Ltd., Jinan 250000, China
    3 College of Life Sciences, Shandong Normal University, Jinan 250014, China
    4 Institute of Advanced Biotechnology and School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China
    5 Center for Advanced Bioindustry Technologies, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    6 Hainan Yazhou Bay Seed Lab, Sanya, Hainan 572024, China
    7 Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China

    These authors contributed equally to the work.
    * Correspondences: Hui Zhao (zhaohui@itbb.org.cn, Dr. Zhao is fully responsible for the distribution of the materials associated with this article); Zhaobo Lang (zblang@psc.ac.cn); Feng Li (lifeng@bellagen.cn)
  • Received:2022-07-17 Accepted:2022-08-10 Online:2022-08-13 Published:2022-10-01

Abstract: A CRISPR/LbCas12a-based nucleic acid detection method that uses crude leaf extracts as samples and is rapid (≤40 min for a full run) and highly sensitive (0.01%) can be used to monitor genetically modified organisms in the field.

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