J Integr Plant Biol. ›› 2024, Vol. 66 ›› Issue (7): 1481-1499.DOI: 10.1111/jipb.13667

• Plant Biotic Interactions • Previous Articles     Next Articles

A vicinal oxygen chelate protein facilitates viral infection by triggering the unfolded protein response in Nicotiana benthamiana

Zhihong Guo, Ning Jiang, Menglin Li, Hongfang Guo, Qi Liu, Xinyu Qin, Zongying Zhang, Chenggui Han and Ying Wang*   

  1. Ministry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China
    These authors contributed equally to this work.
    *Correspondence: Ying Wang (yingwang@cau.edu.cn)
  • Received:2024-01-13 Accepted:2024-03-31 Online:2024-05-02 Published:2024-07-01

Abstract: Vicinal oxygen chelate (VOC) proteins are members of an enzyme superfamily with dioxygenase or non-dioxygenase activities. However, the biological functions of VOC proteins in plants are poorly understood. Here, we show that a VOC in Nicotiana benthamiana (NbVOC1) facilitates viral infection. NbVOC1 was significantly induced by infection by beet necrotic yellow vein virus (BNYVV). Transient overexpression of NbVOC1 or its homolog from Beta vulgaris (BvVOC1) enhanced BNYVV infection in N. benthamiana, which required the nuclear localization of VOC1. Consistent with this result, overexpressing NbVOC1 facilitated BNYVV infection, whereas, knockdown and knockout of NbVOC1 inhibited BNYVV infection in transgenic N. benthamiana plants. NbVOC1 interacts with the basic leucine zipper transcription factors bZIP17/ 28, which enhances their self-interaction and DNA binding to the promoters of unfolded protein response (UPR)-related genes. We propose that bZIP17/28 directly binds to the NbVOC1 promoter and induces its transcription, forming a positive feedback loop to induce the UPR and facilitating BNYVV infection. Collectively, our results demonstrate that NbVOC1 positively regulates the UPR that enhances viral infection in plants.

Key words: beet necrotic yellow vein virus, bZIP17/28, Nicotiana benthamiana, unfolded protein response, VOC1

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