J Integr Plant Biol. ›› 2024, Vol. 66 ›› Issue (10): 2288-2303.DOI: 10.1111/jipb.13752  cstr: 32098.14.jipb.13752

• Plant Biotic Interactions • Previous Articles    

Fusarium graminearum effector FgEC1 targets wheat TaGF14b protein to suppress TaRBOHD-mediated ROS production and promote infection

Shengping Shang1, Yuhan He1, Qianyong Hu1, Ying Fang1,2, Shifeng Cheng1 and Cui‐Jun Zhang1*   

  1. 1. Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
    2. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

    *Correspondence: Cui‐Jun Zhang (zhangcuijun@caas.cn)
  • Received:2024-03-30 Accepted:2024-07-11 Online:2024-08-07 Published:2024-10-01
  • Supported by:
    This work was supported by grants from the Elite Young Scientists Program of the CAAS (110243160001008), the Program for Guangdong “ZhuJiang” Introducing Innovative and Entrepreneurial Teams (2019ZT08N628), and the Shenzhen Science and Technology Program (AGIS‐ZDKY202002).

Abstract: Fusarium head blight (FHB), caused by Fusarium graminearum, is a devastating disease of wheat globally. However, the molecular mechanisms underlying the interactions between F. graminearum and wheat remain unclear. Here, we identified a secreted effector protein, FgEC1, that is induced during wheat infection and is required for F. graminearum virulence. FgEC1 suppressed flg22- and chitin-induced callose deposition and reactive oxygen species (ROS) burst in Nicotiana benthamiana. FgEC1 directly interacts with TaGF14b, which is upregulated in wheat heads during F. graminearum infection. Overexpression of TaGF14b increases FHB resistance in wheat without compromising yield. TaGF14b interacts with NADPH oxidase respiratory burst oxidase homolog D (TaRBOHD) and protects it against degradation by the 26S proteasome. FgEC1 inhibited the interaction of TaGF14b with TaRBOHD and promoted TaRBOHD degradation, thereby reducing TaRBOHD-mediated ROS production. Our findings reveal a novel pathogenic mechanism in which a fungal pathogen acts via an effector to reduce TaRBOHD-mediated ROS production.

Key words: effector, FHB, Fusarium graminearum, reactive oxygen species, wheat

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