J Integr Plant Biol. ›› 2024, Vol. 66 ›› Issue (11): 2362-2378.DOI: 10.1111/jipb.13759  cstr: 32098.14.jipb.13759

• Cell and Developmental Biology • Previous Articles     Next Articles

Coordination of miR319–TaPCF8 with TaSPL14 orchestrates auxin signaling and biosynthesis to regulate plant height in common wheat

Pingan Hao1,2†, Chao Jian2†, Chenyang Hao2†, Shujuan Liu2, Jian Hou2, Hongxia Liu2, Haixia Liu2, Xueyong Zhang2*, Huixian Zhao1* and Tian Li2*   

  1. 1. College of Life Sciences, Northwest A&F University, Yangling 712100, China
    2. State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

    These authors contributed equally to this article.
    *Correspondences: Xueyong Zhang (zhangxueyong@caas.cn); Huixian Zhao (hxzhao212@nwafu.edu.cn); Tian Li (litian@caas.cn, Dr. Li is responsible for the distribution of all materials associated with this article)
  • Received:2024-02-23 Accepted:2024-07-15 Online:2024-08-07 Published:2024-11-01
  • Supported by:
    This research was financially supported by the Innovation Program of Chinese Academy of Agricultural Sciences (CAAS‐CSCB‐202401), the National Natural Science Foundation of China (32172045), and Beijing Natural Science Foundation (6242032).

Abstract: Wheat culms, comprising four to six internodes, are critically involved in determining plant height and lodging resistance, essential factors for field performance and regional adaptability. This study revealed the regulatory function of miR319 in common wheat plant height. Repression of tae-miR319 through short tandem target mimics (STTM) caused an increased plant height, while overexpression (OE) of tae-miR319 had the opposite effect. Overexpressing a miR319-resistant target gene TaPCF8 (rTaPCF8), increased plant height. TaPCF8 acted as a transcription repressor of downstream genes TaIAAs, which interact physically with TaSPL14. The significant differences of indole-3-acetic acid (IAA) contents indicate the involvement of auxin pathway in miR319-mediated plant height regulation. Finally, we identified two TaPCF8 haplotypes in global wheat collections. TaPCF8-5A-Hap2, as per association and evolution examinations, was subjected to strong substantial selection throughout wheat breeding. This haplotype, associated with shorter plant height, aligns with global breeding requirements. Consequently, in high-yield wheat breeding, we proposed a potential molecular marker for marker-assisted selection (MAS). Our findings offer fresh perspectives into the molecular mechanisms that underlie the miR319–TaPCF8 module's regulation of plant height by orchestrating auxin signaling and biosynthesis in wheat.

Key words: auxin, plant height, tae‐miR319, TaIAAs, TaPCF8, TaSPL14, Triticum aestivum

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