J Integr Plant Biol.

• Research Article • Previous Articles    

High-quality genome of allotetraploid Avena barbata provides insights into the origin and evolution of B subgenome in Avena

Qiang He1,2†, Yao Xiao1†, Tao Li1, Yaru Wang1, Yitao Wang1, Yu Wang1, Wei Li1,2, Ningkun Liu1,2, Zhizhong Gong1,3 and Huilong Du1,2*   

  1. 1. College of Life Sciences, Institute of Life Science and Green Development, Hebei University, Baoding 071000, China
    2. Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding 071000, China
    3. State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100094, China

    These authors contributed equally to this work.
    *Correspondence: Huilong Du (huilongdu@hbu.edu.cn)
  • Received:2024-07-01 Accepted:2025-02-24 Online:2025-04-14
  • Supported by:
    This work was supported by the Young Elite Scientists Sponsorship Program by CAST Grant No. YESS20210080, the National Natural Science Foundation of China (Grant No.32100500), the Natural Science Foundation of Hebei Province (Grant Nos: C2021201048 and C2023201074) and the Interdisciplinary Research Program of Natural Science of Hebei University (Grant No: 513201422004). The Excellent Youth Research Innovation Team of Hebei University (Grant No. QNTD202401). Funded by the Science and Technology Project of the Hebei Education Department (Grant No. QN2024271).

Abstract: Avena barbata, a wild oat species within the genus Avena, is a widely used model for studying plant ecological adaptation due to its strong environmental adaptability and disease resistance, serving as a valuable genetic resource for oat improvement. Here, we phased the high-quality chromosome-level genome assembly of A. barbata (6.88 Gb, contig N50 = 53.74 Mb) into A (3.57 Gb with 47,687 genes) and B (3.31 Gb with 46,029 genes) subgenomes. Comparative genomics and phylogenomic analyses clarified the evolutionary relationships and trajectories of A, B, C and D subgenomes in Avena. We inferred that the A subgenome donor of A. barbata was Avena hirtula, while the B subgenome donor was probably an extinct diploid species closely related to Avena wiestii. Genome evolution analysis revealed the dynamic transposable element (TE) content and subgenome divergence, as well as extensive structure variations across A, B, C, and D subgenomes in Avena. Population genetic analysis of 211 A. barbata accessions from distinct ecotypes identified several candidate genes related to environmental adaptability and drought resistance. Our study provides a comprehensive genetic resource for exploring the genetic basis underlying the strong environmental adaptability of A. barbata and the molecular identification of important agronomic traits for oat breeding.

Key words: allotetraploid, Avena barbata, B subgenome, genome evolution, subgenome differentiation

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22