J Integr Plant Biol.

• Brief Communications •    

Interspecies rice versus Arabidopsis thaliana protein–protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing

Jie Huang1†, Yu Cheng1†, Jing Ruan1,2†, Xixi Liu1, Dandan Xia3, Yiting Chen1, Delong Fan1, Jiezheng Ying1, Yifeng Wang1, Xiaohong Tong1, Zhiyong Li1, Dawei Xue2, Jianwei Zhang3, Jian Zhang1* and Yuxuan Hou1*   

  1. 1. State Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China

    2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China

    3. National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China

    These authors contributed equally.

    *Correspondences: Jian Zhang (zhangjian@caas.cn), Yuxuan Hou (houyuxuan@caas.cn, Dr. Houis is fully responsible for the distribution of the materialsassociated with this article)

  • Received:2025-09-16 Accepted:2025-11-13 Online:2025-12-05
  • Supported by:
    This work was supported by Biological Breeding‐National Science and Technology Major Project (Grant No. 2023ZD040662), National Key R&D Program of China (Grant No. 2024YFF1000600), Zhejiang Provincial Natural Science Foundation (Grant No. LQN25C130011, LY23C130004), Natural Science Foundation of China (Grant No. 32472115, U22A20456 and W2412006), Major Program of Natural Science Foundation of Zhejiang (Grant No. LD24C130001), and ASTIP program of CAAS.
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