J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (6): 616-619.

• Research Articles • Previous Articles     Next Articles

Construction of a Trehalose-6-phosphate Synthase Gene Driven by Drought-responsive Promoter and Expression of Drought-resistance in Transgenic Tobacco

ZHAO Hui-Wu, CHEN Yang-Jian, HU Yuan-Lei, GAO Yin, LIN Zhong-Ping   

Abstract:

The trehalose-6-phosphate synthase gene (TPS) from Saccharomyces cerevisiae Hansen and the drought-responsive promoter from Arabidopsis thaliana (L.) Heynh. have been cloned by PCR procedure. A plant expression vector with TPS under control of Prd29A has been constructed and used for the genetic transformation of tobacco. The transgenic tobacco with Prd29A/TSP demonstrated TPS expression with increased drought tolerance under drought stress. Some obvious morphological changes including dwarf and fine shoot, lancet-shaped leaves and vigorous auxiliary buds have been observed in a few transformed plants.

干旱诱导性启动子驱动的海藻糖-6-磷酸合酶基因载体的构建及转基因烟草的耐旱性
赵恢武 陈杨坚 胡鸢雷 高音 林忠平
(北京大学生命科学学院蛋白质工程及植物基因工程国家重点实验室,北京100871)

摘要:通过PCR程序克隆拟南芥(Arabidopsis thaliana (L.)Heynh.)的干旱诱导性启动子Prd29A及来自酿酒酵母(Saccharomyces cerevisiae Hansen)的海藻糖-6-磷酸合酶基因(TPS),并将它们组成可在植物中表达的载体RT。通过根癌土壤杆菌(Agrobacterium tumefaciens (Smithet-Townsend)Conn)LBA4404介导,获得转基因烟草(Nicotiana tabacum L.)。Southern分析表明,TPS基因已经整合到烟草基因组中。Northern分析表明,TPS基因的表达受干旱胁迫的诱导。转基因烟草的形态发生多种改变,包括植株变矮,茎变细,叶子呈柳叶状,腋芽明显,同时耐旱性得到增强。

关键词: 干旱诱导性启动子;海藻糖-6-磷酸合酶基因;烟草;耐旱性

Key words: drought-responsive promoter, trehalose-6-phosphate synthase, Nicotiana tabacum, drought tolerance

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22