J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (11): 1321-1326.

• Research Articles • Previous Articles     Next Articles

Analysis of Genetic Structure of Natural Populations of Castanopsis fargesii by RAPDs

ZHU Qi-Hui, PAN Hui-Xin, ZHUGE Qiang, YIN Tong-Ming, ZOU Hui-Yu and HUANG Min-Ren   

Abstract:

Genetic diversity and population genetic structure in 188 individuals from five natural populations of Castanopsis fargesii Franch. were studied by RAPD markers. Three hundred and eighty five loci were identified with 41 oligonucleotide primers, out of which 157 loci were polymorphic and accounted for 40.78% of total genetic diversity at species level. Shannon's indices of diversity (I) and Nei's gene diversity (h) were 0.459 7 and 0.296 at the species level, respectively. The result showed that genetic variation of C. fargesii populations mainly existed within populations. Genetic differentiation (Hsp Hpop)/Hsp estimated with Shannon's index of diversity and coefficient of gene differentiation (Gst) were 0.0476 and 0.042 9 respectively, which were confirmed by the analysis of molecular variance (AMOVA). Therefore, it is apparent that within population variation accounted for 94.97% and among populations variation accounted for only 5.03% of the total genetic diversity. AMOVA also indicated that there was significant differentiation among populations as well as among individuals within a population.

栲树天然群体遗传结构的RAPD分析
朱其慧 潘惠新 诸葛强 尹佟明 邹惠渝 黄敏仁*

(南京林业大学林木遗传和基因工程重点实验室,南京210037)

摘要: 利用RAPD分子标记对 5个栲树 (CastanopsisfargesiiFranch .)天然群体共计 188个个体的遗传多样性和群体遗传结构进行了分析。 4 1个随机寡核苷酸引物共检测到 385个位点 ,其中多态位点 15 7个 ,占 4 0 .78%。物种水平的Shannon多样性指数I=0 .4 5 97,Nei基因多样度h =0 .2 96。遗传变异分析表明 ,栲树群体的遗传变异主要存在于群体内 ,利用Shannon多样性指数估算的分化 (Hsp-Hpop) /Hsp=0 .0 4 76 ,遗传分化系数Gst =0 .0 4 2 9,分子方差分析 (AMOVA)也证实了这一结论 ,群体内的变异组分占了 94 .97% ,群体间变异只占 5 .0 3%。AMOVA分析结果的显著性检验也表明 ,群体间及群体内个体间均呈现出显著分化 (P <0 .0 0 1)。
 

关键词: 栲树;天然群体;RAPD;遗传结构

Key words: Castanopsis fargesii, natural populations, RAPD, genetic structure

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