J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (11): 1327-1332.

• Research Articles • Previous Articles     Next Articles

Genetic Diversity of Hordeum bogdanii Wilensky Native to Xinjiang, China, Based on STS-PCR Markers

GUO Hong, WEI Yu-Ming, CHEN Fang and ZHENG You-Liang   

  • Published:2002-11-21

Abstract:

The genetic diversity among 32 accessions ofHordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS-PCR primer sets derived from RFLP clones of the wheat (Triticum aestivum L.) or barley (Hordeum vulgare L.) mapping. Out of the 22 STS-PCR markers, only three markers gave products which did not generate polymorphic bands upon digestion with HinfⅠ, HhaⅠ, HaeⅢ and RsaⅠ, while 19 out of 22 markers (86.4%) and 46 out of 88 marker/enzyme combinations (52.3%) revealed polymorphisms. Among the 32H. bogdanii accessions, a total of 315 bands were observed in 88 STS-PCR marker/enzyme combinations, with 3.6 bands each. One hundred and twenty three out of 315 bands (39.0%) were polymorphic, among which 1 to 6 polymorphic bands were generated by each polymorphic marker/enzyme combination. The STS-PCR based genetic diversity index (GD) among 32 H. bogdanii accessions ranged between 0.078 to 0.352, with a mean of 0.198. Based on the GD matrix, a dendrogram showing the geneticrelationships between accessions was constructed using the unweighted pair group method with arithmetic average (UPGMA). Results showed that all 32 accessions could be distinguish hed by STS-PCR markers. The accessions originated from the same region were distributed within different groups or subgroups. This study indicates that the genetic diversity of H. bogdanii is not closely correlated with the geographical distribution.

应用STS-PCR 标记研究新疆布顿大麦的遗传多样性
郭 红1,2 魏育明2* 陈 放1  郑有良2*

(1.  四川大学生命科学学院,成都610064;2.  四川农业大学小麦研究所,都江堰611830)

摘要: 利用22个来源于小麦(Triticum aestivum L.)和栽培大麦(Hordeum vulgare L.)的STS-PCR标记,研究了32份新疆布顿大麦(Hordeum bogdanii  Wilensky)的遗传多样性.在这22个STS-PCR标记中,仅有3个标记的扩增产物经HinfⅠ、HhaⅠ、HaeⅢ和RsaⅠ 4种限制性内切酶消化后没有产生多态性DNA片段,而19个标记(占86.4%)和46种标记/酶组合(占52.3%)能够揭示材料间的多态性.在32份布顿大麦材料的88种STS-PCR标记/酶组合中,总共得到315条DNA片段,平均每个标记/酶组合能得到3.6条DNA片段.在这315条DNA片段中,共有123条片段(占39.0%)具有多态性,每一个多态性标记/酶组合能获得1~6条多态性DNA片段.STS-PCR标记揭示的32份布顿大麦的遗传距离变化范围为0.078~0.352,平均为0.198.根据STS-PCR标记的遗传距离矩阵,采用不完全加权算术平均数法(UPGMA)构建了32份布顿大麦群体间的遗传关系树状图,结果表明STS-PCR标记能将32份材料完全区分开来.同时,来源于同一地方的不同居群没有明显地聚类在一起,表明新疆布顿大麦的遗传多样性与其地理分布相关不紧密.

关键词: 布顿大麦;STS-PCR 标记;遗传多样性;遗传关系

通讯作者。-MAIL: Ymwei @ sc.homeway.com.cn; ymwei @ sicau.edu.cn

Key words: Hordeum bogdanii;STS-PCR marker, genetic diversity;genetic relationship

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