J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (5): 557-561.

• Research Articles •

Comparison of Phycobiliproteins from Gracilaria lemaneiformis (Rhodophyceae) and Its Pigment Mutants in Spectral and Molecular Respects

SUI Zheng Hong, ZHANG Xue Cheng*, CHENG Xiao Jie

• Published:2002-05-15

Abstract:

Comparative studies of absorption spectra of phycobiliproteins of Gracilaria lemaneiformis Greville and its pigmental mutants were conducted in this study. The results showed that the absorption spectra of phycoerythrins (PE) from different material changed significantly, while those of phycocyanins(PC)and allophycocyanins (APC) were basically similar. In order to disclose the essence of the difference, partial sequences of the subunit genes of PE of Qingdao strain of G. lemaneiformis (qd) and its pigmental mutants were determined. The amino acid sequences were deduced and used to explain spectral shifts of PE from the pigmental mutants. The amino acid sequences of PE resembled each other, and several residues changed among qd and its pigmental mutants. Residue substitutions were found in a region consisting of amino acids which determined the secondary structure and subunits interactions, thus might influence the confirmation and interaction of subunits, and further caused spectral deviation.

（青岛海洋大学海洋生命学院，青岛266003）

!通讯作者。E-mail:xczhamg @ ouqd.edu.cn.

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