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J Integr Plant Biol, 2003, 45 (11): 1307-1311, Research Article
Improvement of Copper-inducible Gene Expression System for Plant
PENG Xiang-Lei, ZHONG Jin, LIANG Bin, HU Yuan-Lei, GAO Yin, LIN Zhong-Ping*
doi:
Abstract

The copper-regulated gene expression system has been developed to control spacial and temporal expression of transgene in plant. It comprises two parts: (1) ace1 gene encoding copper-respon-sive transcription factor under the control of a constitutive or organ-specific promoter, and (2) a gene of interest under the control of a chimeric promoter consisting of the CaMV 35S (-90 to +8) promoter linked to the metal responsive element (MRE) carrying activating copper-metallothionein expression (ACE1)-binding sites. Here, the effectiveness of two different ACE1-binding cis -elements which derive from 5''-regulatory region of yeast metallothionein gene was investigated in transgenic tobacco (Nicotiana tabacum L. cv. W38). The results revealed that the MRE (-210 to -126) could increase the system inducibility by 50% – 100% compared with the previously reported MRE (-148 to -105). It is potential to use the copper-inducible system to control valuable gene traits in plant biotechnology.

用于植物的铜诱导基因表达系统的改进
彭向雷 钟 瑾 梁 斌 胡鸢雷 高 音 林忠平
(北京大学生命科学学院蛋白质工程及植物基因工程国家重点实验室,北京1 0 0 8 7 1 )


摘要: 铜诱导基因表达系统已被用于植物转基因表达的时空调控。它由两部分构成:(1)组成型或器官专一性表达的ace1基因编码铜反应性的转录因子;(2)融合启动子控制下的目的基因,融合启动子由含ACE1结合位点的金属反应元件(MRE)连接CaMV 35S (-90-+8) 启动子组成。本实验测试了来自酵母金属硫蛋白基因5′调控区的两个不同的ACE1结合区域在转基因烟草(Nicotiana tabacum L.cv.W38)中的效果。结果表明,与MRE(-148~-105)相比,使用MRE(-210~-126)的铜诱导系统效率增加50%到100%。在植物生物技术中使用这一系统控制基因状是很有潜力的。
关键词: 铜诱导系统;金属反应元件;金属反应转录因子;转基因烟草

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