J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (5): 530-535.

• Research Articles •

Phylogenetic Relationship of an Extremely Endangered Species, Pinus squamata (Pinaceae) Inferred from Four Sequences of the Chloroplast Genome and ITS of the Nuclear Ribosomal DNA

ZHANG Zhi-Yong, YANG Jun-Bo, LI De-Zhu

• Published:2003-05-15

Abstract:

Pinus squamata X. W. Li, a recently-described species of the Pinaceae, is an extremely endangered pine with only 32 individuals in the wild. This species was thought to be intermediate between P. bungeana Zucc. ex Endl. of subgen. Strobus and P. yunnanensis Franch. of subgen. Pinus by the original author. We made an effort to address the question of the phylogenetic relationship of this peculiar and important species in the context of the major groups of the genus Pinus by using sequences of the rbc L, mat K genes, rpl20-rps18 spacer, trn V intron of the chloroplast genome and the nuclear ribosomal ITS region. The results of the separated analysis and the combined analysis of the four cpDNA sequences and ITS sequence indicated that P. squamata was a stable member of subsect. Gerardianae and P. gerardiana Wall. was the closest species of P. squamata phylogenetically. As a result, the causes of the distribution pattern of subsect. Gerardianae were also discussed.

（1. 中国科学院昆明植物研究所生物多样性与生物地理开放实验室，昆明650204；
2. 江西农业大学农学院，南昌330045）

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