J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (5): 614-620.

• Research Articles •

### Rapid Constructing a Genetic Linkage Map by AFLP Technique and Mapping a New Gene]tms5

JIA Jian-Hang, LI Chuan-You, DENG Qi-Yun, WANG Bin

• Published:2003-05-15

Abstract:

In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1 537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was tigged and mapped onto chromosome 2 during the development of the linkage map.

（中国科学院遗传与发育生物学研究所，北京!100101）

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