J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (7): 795-801.

• Research Articles • Previous Articles     Next Articles

Environmental Stresses and Redox Status of Ascorbate

JIN Yue-Hua, TAO Da-Li, HAO Zhan-Qing, YE Ji, DU Ying-Jun, LIU Hai-Ling, ZHOU Yong-Bin   

  • Published:2003-07-15

Abstract:

To investigate the effects of environmental stresses on ascorbic acid content and its redox status, the effects of freezing and drought on ascorbate and dehydro-ascorbate content and activities of four enzymes involved in the ascorbate-glutathione cycle in some conifers were studied. The results showed that both freezing and drought induced the decrease in ascorbate content and the increase in dehydro-ascorbate content. The activities of ascorbate peroxidase (APX) and monodehydro-ascorbate reductase (MDAR) were decreased by freezing stress. At the beginning of exposure to air, water loss from detached needles induced the increase in the activities of APX and MDAR. Further water loss turned to decrease the APX and MDAR activities. The activities of dehydro-ascorbate reductase (DHAR) and glutathione reductase (GR) were not sensitive to changes in temperature and water content of the needles. It is concluded that moderate tempera-ture or water stresses may induce the acclimation and increase in the ability of the H2O2 scavenging system, while strong stresses decrease the ability and induce injury of plant tissues. Correlation between ascorbate content and activities of related enzymes and cold tolerance of conifers were also reported.

环境肋迫和抗血酸的氧化还原状态

靳月华 陶大立 郝占庆 叶 吉 杜英君 刘海玲 周永斌

(1.中国科学院沈阳应用生态研究所陆地生态过程开放实验室,沈阳110016; 2.沈阳农业大学,沈阳110161)

摘要: 为了解环境胁迫对植物体中抗坏血酸含量及氧化还原状态的影响,以不同强度的冰冻和干旱两种胁迫为例,研究了它们对沈阳几种针叶树离体叶抗坏血酸、脱氢抗坏血酸含量以及抗坏血酸-谷胱甘肽循环中4种酶活性的影响.结果表明,两种胁迫达到一定强度后,都能使还原态抗坏血酸含量下降而使脱氢抗坏血酸含量上升.冰冻使抗坏血酸过氧化酶和单脱氢抗坏血酸还原酶活性下降.轻度失水使这两种酶活性上升,失水加重后转而趋于下降.脱氢抗坏血酸还原酶和谷胱甘肽还原酶活性对两种胁迫反应均不如前两种酶敏感.结合以前的研究结果,认为这一H2O2清除系统在导致驯化(acclimation)的轻度胁迫作用下可以得到加强,而当胁迫强度过大时则其清除能力下降并使组织受到伤害.文中还报告了沈阳几种针叶树抗寒性和针叶中抗坏血酸含量及上述4种酶活性之间的相关关系.

关键词: 冰冻;干旱; 抗坏血酸;抗坏血酸-谷胱甘肽代谢途径

通讯作者。E-mail: taodali @ iae.ac.cn.

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