J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (7): 809-814.

• Research Articles • Previous Articles     Next Articles

Different Responses of Camptothecin and 10-Hydroxycamptothecin to Heat Shock in Camptotheca acuminata Seedlings

ZU Yuan-Gang, TANG Zhong-Hua, YU Jing-Hua, LIU Shi-Gang, WANG Wei, GUO Xiao-Rui   


Plants interact with their environment by producing a diverse array of secondary metabolites, one of which is alkaloid. In this study, alkaloids, including camptothecin (CPT) and 10-hydroxycamptothecin (HCPT), malondialdehyde (MDA) and chlorophyll contents were measured during heat shock in seedlings of Camptotheca acuminata Decaisne unique to China. Responses of different tissues, including young leaves, old leaves, buds and barks, to heat shock were examined in alkaloid changes. CPT and HCPT concentra-tions reached their peak values separately at 38 ℃ and 40 ℃, which were below the lethal heat-shock temperature indicated by MDA and chlorophyll, and their great changes took place in young leaves. These results indicated that CPT and HCPT were involved in the C. acuminata resistance against heat shock from its environment. Furthermore, plant rigidly observed the cost-benefit principle and mobilized and allocated limited alkaloid sources to young and reproductive tissues preferentially. In addition, HCPT dis-played well-regulated changes during incubation at sublethal temperature, and this indicated that HCPT might play a more positively defensive role in enhancement of plant thermotolerance than CPT does.

唐中华 于景华 刘士刚 王 微 郭晓瑞
(东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040)

摘要: 植物在长期的生态环境适应过程中,产生了包括生物碱在内的大量次生代谢物。本文以我国特有树种——喜树(Camptotheca acuminata Decaisne)为材料,研究其不同器官中喜树碱(camptothecin, CPT)和10- 羟基喜树碱(10-hydroxycamptothecin, HCPT)在不同热激温度和时间情况下的含量变化。CPT和HCPT 变化呈现出较好的相互消长关系,并且分别在38 ℃和40 ℃达到各自的峰值,比以丙二醛和叶绿素为指标的致死温度低了2~4℃; HCPT 在热激过程中的变化较CPT 活跃;极易受到攻击和伤害的嫩叶中的生物碱含量变化最大。由此推断,CPT 和HCPT 遵循“幼嫩和生殖器官优先保护”的原则,从而有效地缓解了高温胁迫,并且HCPT 和CPT代表了不同的防御策略。
关键词: 喜树;喜树碱;10-羟基喜树碱;响应;热激

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