J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (7): 864-870.

• Research Articles • Previous Articles     Next Articles

Cloning and Analysis of a Disease Resistance Gene Homolog from Soybean

WANG Bang-Jun, ZHANG Zhi-Gang, LI Xue-Gang, WANG Yong-Jun, HE Chao-Ying, ZHANG Jin-Song, CHEN Shou-Yi   

Abstract:

Conserved domains e.g. nucleotide binding site (NBS) were found in several cloned plant disease resistance genes. Based on the NBS domain, resistance gene analogs (RGAs) have been isolated previously and were used as probes to screen a soybean (Glycine max L. Merr.) cDNA library. A full-length cDNA, KR3, was obtained by screening the library and rapid amplification of cDNA ends (RACE) method. Sequence analysis revealed that the cDNA is 2 353 bp in length and the open reading frame (ORF) codes for a polypeptide of 636 amino acids with a Toll-Interleukin-1 receptor (TIR) and a NBS domain. Sequence alignment showed that it was similar to N gene of tobacco. The phylogenetic tree analysis of R proteins with NBS from higher plants was performed. The KR3 gene has low copies in soybean genome and its expression was induced by exogenous salicylic acid (SA).

大豆抗病基因同源序列的克隆与分析
王邦俊  张志刚  李学刚  王永军  贺超英  张劲松  陈受宜
(1. 中国科学院遗传与发育生物学研究所植物生物技术开放实验室,北京 100101;
2.西南农业大学中心实验室,重庆 400716)

摘要: 已克隆的植物抗病基因序列存在一些相对保守的结构区域。利用根据核苷酸结合位点(NBS)结构域扩增所获得的大豆抗病基因同源片段为混合探针,进行大豆cDNA 文库筛选。通过筛库和5′RACE-PCR 扩增后,获得一全长基因KR3。KR3 的长度为2353 bp,编码636 个氨基酸。KR3 蛋白在结构上与烟草抗花叶病毒N 基因蛋白有较高的同源性,具有Toll/ 白细胞介素-1 受体(TIR)、NBS 等抗病基因的分子特征。Southern 杂交显示KR3 在基因组中为低拷贝;RT -PCR 分析表明, 该基因的表达受外源水杨酸的诱导。
关键词: 抗病基因同源序列;核苷酸结合位点;Toll/ 白细胞介素-1 受体;大豆

通讯作者。 E-mail: <sychen@genetics.ac.cn;jszhang@genetics.ac.cn>。

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