J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (7): 871-877.
• Research Articles •
PAN Qing-Hua, HU Zhen-Di, Tanisaka TAKATOSHI, WANG Ling
The gene Pi15 for resistance of rice to Magnaporthe grisea was previously identified as being linked to the gene Pii. However, there is a debate on the chromosomal position of the Pii gene, because it was originally mapped on chromosome 6, but recent work showed it might be located on chromosome 9. To determine the chromosomal location of the Pi15 gene, a linkage analysis using molecular markers was performed in a F2 mapping population consisting of 15 resistant and 141 susceptible plants through bulked-segregant analysis (BSA) in combination with recessive-class analysis (RCA). Out of 20 microsatellite markers mapped on chromosomes 6 and 9 tested, only one marker, RM316 on chromosome 9, was found to have a linkage with the Pi15 gene with a recombination frequency of (19.1 ± 3.7)%. To confirm this finding, four sequence-tagged site (STS) markers mapped on chromosome 9 were tested. The results suggested that marker G103 was linked to the Pi15 gene with a recombination frequency of (5.7 ± 2.1)%. To find marker(s) more closely linked to the Pi15 gene, random amplified polymorphic DNA (RAPD) analysis was performed. Out of 1 000 primers tested, three RAPD markers, BAPi15486, BAPi15782 and BAPi15844 were found to tightly flank the Pi15 gene with recombination frequencies of 0.35%, 0.35% and 1.1%, respectively. These three RAPD markers should be viewed as the starting points for marker-aided gene pyramiding and cloning. A new gene cluster of rice blast resistance on chromosome 9 was also discussed.
潘庆华 胡珍娣 谷坂隆俊 王 玲
（1. 华南农业大学资源环境学院植物抗病遗传学研究室，广州 5 1 0 6 4 2；
2. Laboratory of Plant Breeding, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan)
PAN Qing-Hua, HU Zhen-Di, Tanisaka TAKATOSHI, WANG Ling. Fine Mapping of the Blast Resistance Gene Pi15, Linked to Pii,[J]. J Integr Plant Biol., 2003, 45(7): 871-877.
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