Abstract:

Two new triterpenoids, 3β, 15α, 21β, 23-tetrahydroxy-12-oleanen-28-oic acid (1), 3β, 6α, 21β, 23-tetrahydroxy-12-oleanen-28-oic acid (2), together with seven known compounds, viz., paeonol (3), 3, 4-dihydroxybenzoic acid (4), scosoletin (5), anthraquinones chrysophanol (6), 4H-1-benzopyran-4-one, 5, 7-dihydroxy-2-methyl (7), β-sitosterol (8) and stigmasterol glucoside (9) were isolated by the chromatography of the silica gel, RP-18 and Sephadex-LH 20 from the EtOAc extract of Neonauclea sessilifolia (Roxb.) Merr. ( Rubiaceae ). Their structures were elucidated based on spectral analysis including 1D-, 2D-NMR (HMQC, HMBC), IR and EIMS. Among them, compound 6 was shown to possess inhibitory activity on the growth of Mycobacterium tuberculosis (Zopf) Lehmann et Neumann with a minimum inhibitory amount of 25 礸, compounds 2 and 4 also showed weak inhibitory activities on the growteof M. tuberculosis.

   无柄新乌檀中的三萜

康文艺 李国红 郝小江

（1.中国科学院昆明植物研究所植物化学与西部资源持续利用国家重点实验室，昆明确650204；2.云南大学工业微生物发酵工程重点实验室，昆明 650091）

摘要：采用活性跟踪的方法对无柄新乌檀(Neonauclea sessilifolia (Roxb.)Merr.)枝干的乙醇提取物进行结核杆菌活性检测.利用硅胶柱层析、Sephadex-LH 20和反相硅胶RP18柱层析,从具有活性的乙酸乙酯部分分得9个化合物,利用一维、二维核磁共振技术(HMQC,HMBC)并结合质谱与红外光谱,将它们鉴定为3 β,15 α,21 β,23-tetrahydroxv-12-oleanen-28-oic acid(1)、3 β,6 α,21 β,23-tetrahydroxy-12-oleanen-28-oic acid(2)、单皮酚(3)、3,4-二羟基苯甲酸(4)、东莨菪内酯(5)、蒽醌大黄酚(6)、5,7-二羟基-2-甲基吡喃酮(7)、β-谷甾醇(8)和^5,22△-豆甾醇-β-D-葡萄糖苷(9).其中化合物1和2是两个新化合物.体外抗结核杆菌试验表明,化合物6对结核杆菌(Mycobacterium tuber-culosis(Zopf)Lehmann et Neumann)具有一定的抑制效果,用量在2μg时出现抑菌圈;化合物2与4具有微弱的抑制活性.

关键词：无柄新乌檀；结核杆菌；三萜

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