J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (3): 330-348.DOI: 10.1111/jipb.12875

Special Issue: Abiotic stress

• Plant-abiotic Interactions • Previous Articles     Next Articles

Polyunsaturated linolenoyl‐CoA modulates ERF‐VII‐mediated hypoxia signaling in Arabidopsis

Ying Zhou1†, Wei-Juan Tan1†, Li-Juan Xie1, Hua Qi1, Yi-Cong Yang1, Li-Ping Huang1, Yong-Xia Lai1, Yi-Fang Tan1, De-Mian Zhou1, Lu-Jun Yu1, Qin-Fang Chen1, Mee-Len Chye2 and Shi Xiao1*   

  1. 1State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat‐Sen University, Guangzhou 510275, China
    2School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong 999077, China

    These authors contributed equally to this work.
    Email: Shi Xiao (xiaoshi3@mail.sysu.edu.cn)
  • Received:2019-09-30 Accepted:2019-10-03 Online:2019-10-08 Published:2020-03-01


In plants, submergence from flooding causes hypoxia, which impairs energy production and affects plant growth, productivity, and survival. In Arabidopsis, hypoxia induces nuclear localization of the group VII ethylene‐responsive transcription factor RELATED TO AP2.12 (RAP2.12), following its dissociation from the plasma membrane‐anchored ACYL‐COA BINDING PROTEIN1 (ACBP1) and ACBP2. Here, we show that polyunsaturated linolenoyl‐CoA (18:3‐CoA) regulates RAP2.12 release from the plasma membrane. Submergence caused a significant increase in 18:3‐CoA, but a significant decrease in 18:0‐, 18:1‐, and 18:2‐CoA. Application of 18:3‐CoA promoted nuclear accumulation of the green fluorescent protein (GFP) fusions RAP2.12‐GFP, HYPOXIA‐RESPONSIVE ERF1‐GFP, and RAP2.3‐GFP, and enhanced transcript levels of hypoxia‐responsive genes. Plants with decreased ACBP1 and ACBP2 (acbp1 ACBP2‐RNAi, produced by ACBP2 RNA interference in the acbp1 mutant) had reduced tolerance to hypoxia and impaired 18:3‐CoA‐induced expression of hypoxia‐related genes. In knockout mutants and overexpression lines of LONG‐CHAIN ACYL‐COA SYNTHASE2 (LACS2) and FATTY ACID DESATURASE 3 (FAD3), the acyl‐CoA pool size and 18:3‐CoA levels were closely related to ERF‐VII‐mediated signaling and hypoxia tolerance. These findings demonstrate that polyunsaturation of long‐chain acyl‐CoAs functions as important mechanism in the regulation of plant hypoxia signaling, by modulating ACBP–ERF‐VII dynamics.

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