Integrative Biology Journals
JIPB

J Integr Plant Biol. ›› 1993, Vol. 35 ›› Issue (9): -.

• Research Articles •    

Gallus Regeneration from Protoplasts of Sugar Beet(Beta vulgaris)

Shao Ming-wen and Ma Long-biao   

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Abstract: Conditions appropriate for isolation and culture of protoplasts from cell suspension cultures of sugar beet (Beta vulgaris L. ) were investigated. Protoplasts with high yields and high quality were obtained by treating cells with a mixture of cellulase, macerozyme R-10 and driselase, or other enzyme combinations. Protoplasts were cultured in MS liquid medium or solid agar medium. Callus was obtained from the cultured protoplasts.

Key words: Sugar beet (Beta vulgaris), Protoplast culture, Callus regeneration

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