J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (5): -.
• Research Articles •
Qiu De-bo, Lu Feng and Xie Shi-wen
Abstract: The enzyme-squash technique had been employed to study the development of megaspores and embryo sacs from tropical plants of Hevea brasiliensis Muel-Arg. and Carica papaya L. Ovules fixed with FAA or FPA were enzymatieally macerated in 3% pectinase cellulase solution or in 3–4% snailase solution for 4–6 h, at 28–30℃ to isolate the embryo sacs after being squashed. The whole structural images of megaspores and embryo sacs at different developmental stages were observed and identified without staining under Olympus BH-2 phase contrast microscope or light microscope after ron-aceto-carmine staining.
Key words: Isolation of embryo sac, Enzyme-squash technique, Hevea brasiliensis, Carica papaya
Qiu De-bo, Lu Feng and Xie Shi-wen. Embryo Sacs of Hevea brasiliensis and Carica papaya Isolated with Enzyme-Squash Technique[J]. J Integr Plant Biol., 1991, 33(5): -.
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