J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (5): -.

• Research Articles •    

Embryo Sacs of Hevea brasiliensis and Carica papaya Isolated with Enzyme-Squash Technique

Qiu De-bo, Lu Feng and Xie Shi-wen   

Abstract: The enzyme-squash technique had been employed to study the development of megaspores and embryo sacs from tropical plants of Hevea brasiliensis Muel-Arg. and Carica papaya L. Ovules fixed with FAA or FPA were enzymatieally macerated in 3% pectinase cellulase solution or in 3–4% snailase solution for 4–6 h, at 28–30℃ to isolate the embryo sacs after being squashed. The whole structural images of megaspores and embryo sacs at different developmental stages were observed and identified without staining under Olympus BH-2 phase contrast microscope or light microscope after ron-aceto-carmine staining.

Key words: Isolation of embryo sac, Enzyme-squash technique, Hevea brasiliensis, Carica papaya

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