Expression of Bovine Growth Hormone Gene in Transgenic Potato

Abstract

Abstract: The expression vector for the bovine growth hormone (bGH) cDNA was constructed. It was introduced into Agrobacteriurn turnefaciens strain LAB4404 by direct transformation. Using the modified leaf-disc co-cultivated method, the bGH gene was transformed into potato (Solanurn tuberosum). Potato tuber pieces started to form shoots on shoot-inducing medium containing kanamycin after infected by A. turnefaciens. Regenerated potato tubers were obtained on sucrose enriched medium. NPT Ⅱ assay, PCR and Southern blot analyses showed that the bGH gene has been integrated into genome of potato. RNA dot blot and Western blot indicated that the bGH gene was transcripted and expressed in transgenic plants.

Key words: Potato, Bovine growth hormone cDNA, Expression

Zhao Qian, Ao Guang-ming, Liu Shu-lan and Han Bi-wen. Expression of Bovine Growth Hormone Gene in Transgenic Potato[J]. J Integr Plant Biol., 1995, 37(11): -.

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Expression of Bovine Growth Hormone Gene in Transgenic Potato

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