Abstract: The changes of stomatal behaviors and functions of grape and cherry cultured in vitro and in nutrition bags were compared through observation of leaf imprints. The results showed that the leaves of grape and cherry grown in vitro in high humidity when treated with factors inducing stomatal closure, all stomata of grape leaf were open. but partly closed in cherry leaf. After acclimatization the stomata volumes of grape and cherry leaves in nutrition bags reduced in various degrees, the average diameter and open index decreased obviously. The surface structure of stomata observed under scanning electron microscope revealed that the stomata of grape and cherry cultured in vitro were roughly at the same level with leaf surface but the stomata of grape in nutrition bags sunk in a little from the leaf surface, while the stomata of cherry leaf obviously raised above the surface. As the stomatal behavior and function of the two crops in vitro were different, it seemed that different humidities were required in transplantation of grape and cherry.

Key words: In vitro, Grape, Cherry, Stomata