J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (10): 961-972.DOI: 10.1111/j.1744-7909.2009.00868.x

Special Issue: Plant Signal Transduction

• Molecular Ecology and Evolution • Previous Articles     Next Articles

Induction of Protection against Paraquat-induced Oxidative Damage by Abscisic Acid in Maize Leaves is Mediated through Mitogen-activated Protein Kinase

Hai-Dong Ding1,2†, Xiao-Hua Zhang1†, Shu-Cheng Xu3† , Li-Li Sun1, Ming-Yi Jiang1,4*, A-Ying Zhang1 and Yin-Gen Jin2   

  1. 1 College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
    2 College of Biosciences and Biotechnology, Yangzhou University, Yangzhou 225009, China
    3 College of Life Sciences, Fuyang Teachers College, Fuyang 236032, China
    4 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-02-27 Accepted:2009-07-13 Published:2009-09-23
  • About author:
    *Author for correspondence Tel: +86 25 8439 6372; Fax: +86 25 8439 6542; E-mail: myjiang@njau.edu.cn

Abstract:

Mitogen-activated protein kinase (MAPK) cascade has been shown to be important components in stress signal transduction pathway. In the present study, protection of maize seedlings (Zea mays L.) against paraquat generated oxidative toxicity by abscisic acid (ABA), its association withMAPK and ZmMPK5, a candidate forMAPK were investigated. Treatment of maize leaves with exogenous ABA led to significant decreases in the content of malondialdehyde, the percentage of ion leakage and the level of protein oxidation (in terms of carbonyl groups) under paraquat (PQ) stress. However, such decreases were blocked by the pretreatment with two MAPK kinase inhibitors PD98059 and U0126. The damage caused by PQ was further aggravated by inhibitors. Two inhibitors also suppressed the total activities of the antioxidant enzymes superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11), and glutathione reductase (GR, EC 1.6.4.2). Besides, treatment with PQ stimulated the activation of a 46 kDa MAPK, which was identified as ZmMPK5 by in-gel kinase assay with immunoprecipitation. These results reveal that ABA-induced protection against PQ-generated oxidative damage is mediated through MAPK cascade inmaize leaves, in which ZmMPK5, a candidate for MAPK, is demonstrated to be involved.

Ding HD, Zhang XH, Xu SC, Sun LL, Jiang MY, Zhang AY, Jin YG (2009). Induction of protection against paraquat-induced oxidative damage by abscisic acid in maize leaves is mediated through mitogen-activated protein kinase. J. Integr. Plant Biol. 51(10), 961–972.

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