J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (7): 663-674.DOI: 10.1111/j.1744-7909.2009.00834.x

• Plant-environmental Interactions • Previous Articles     Next Articles

Regulation of OsSPX1 and OsSPX3 on Expressions of OsSPX domain Genes and Pi-starvation Signaling in Rice

Zhiye Wang, Han Hu, Hongjie Huang, Ke Duan, Zhongchang Wu and Ping Wu*   

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China )
  • Received:2009-02-25 Accepted:2009-03-25 Published:2009-07-10
  • About author: *Author for correspondence. Tel(Fax): +86 571 8820 6617; E-mail: clspwu@zju.edu.cn


The rice (Oryza sativa L.) genome contains at least six genes exclusively with an SPX (SYG1/PHO81/XPR1) domain at the N-terminal, designated as OsSPX1-6. Here we report the diverse expression patterns of the OsSPX genes in different tissues and their responses to Pi-starvation. Among them, five genes, OsSPX1, 2, 3, 5 and 6 are responsive to Pi-starvation in shoots and/or in roots. The subcellular localization analysis indicates that OsSPX1 and OsSPX2 is exclusively located in nucleus, OsSPX3 in the cytoplasm, and OsSPX4 is a membrane localization protein. OsSPX1 regulates OsSPX2, 3 and 5 at the transcription level and is positively involved in the responses of the genes to Pi-starvation. Overexpression of OsSPX3 downregulates OsSPX5 in shoots under Pi-sufficiency. OsSPX3 negatively regulates the PSI (Pi starvation induced) gene, OsIPS1 and is involved in the responses of miR399 and OsPHO2 to Pi-starvation. Our results suggest that OsSPX1 may be a regulator involved in the transcriptions of OsSPX2, 3 and 5. OsSPX3 plays a role in OsIPS1/miR399 mediated long distance regulation on OsPHO2. Our results also indicate that OsSPX3 is involved in plant tolerance to Pi-starvation stress.

Wang Z, Hu H, Huang H, Duan K,Wu Z,Wu P (2009). Regulation of OsSPX1 and OsSPX3 on expression of OsSPX domain genes and Pi-starvation signaling in rice. J. Integr. Plant Biol. 51(7), 663-674.

Key words: Oryza sativa L., SPX domain gene, Expression regulation, Pi-signaling

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