Abstract:

A new inducible Cre/lox system was constructed in transgenic tobacco (Nicotiana tabacum L.) plants. The inducer-treatment of tobacco callus mediates an excision event in which the selectable marker gene and Cre gene between two lox sites were deleted. A chloroacetanilide-induced promoter (In5-2) was used to control the expression of Cre gene in this system. Molecular analysis of transgenic tobacco plants showed the interested gene, b-glucuronidase (gus), was integrated into the genome whether removing has been successful, and forty-five out of forty-eight T₀ plants were transgenic tobacco without the marker gene, hpt. This system uses a single vector to circumvent the flaw of other dual recombinase vector systems.

Key words: chloroacetanilide-induced promoter (In5-2), Cre/lox, marker-free, transgenic tobacco