J Integr Plant Biol. ›› 1994, Vol. 36 ›› Issue (11): -.
• Research Articles •
Liang Xiao-you, Mi Jing-jiu, Zhu Yu-xian and Chen Zhang-liang
By in situ hybridization of bacterium clone and analysis of restriction enzyme digestion, both CMV-cp gene and Bt-toxin gene were inserted one by one into T-DNA of binary plant expression vector pea. The reconstructed plasmid was named pE14. Then, tomato was transformed with pE14 mediated by Agrobacterium tumefaciens GV311-SE, four regenerated tomato plants were obtained on the MS medium containing 100 μg/mL kanamycin. Assay of nopaline, dot blotting of tomato genomic DNA and PCR amplication of CMV-cp gene and Bt-toxin gene from genomic DNA showed that CMV-cp gene and Bt-toxin gene were transferred into the four regenerated tomato plants simultaneously with T-DNA, and no recombination of genes occurred. RNA dot blotting showed that two of them could express simultaneously the CMV-cp gene and Bt-toxin gene proteins. The resistances to virus and insect of the transgenic tomato plants will be tested in their F1 and F2 regenerations.
Construction of plant expression vector,
Transformation of tomato,
Expression of foreign genes
Liang Xiao-you, Mi Jing-jiu, Zhu Yu-xian and Chen Zhang-liang. Construction of Plant Expression Vector with Double Resistance to Virus and Insect and Identification of Transformation in Tomato[J]. J Integr Plant Biol., 1994, 36(11): -.
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