J Integr Plant Biol. ›› 2004, Vol. 46 ›› Issue (1): 93-99.

• Research Articles • Previous Articles     Next Articles

Cloning and Expression Analysis of the B Subunit of V-H+-ATPase in Leaves of Halophyte Suaeda salsa Under Salt Stress

LI Ping-Hua, WANG Zeng-Lan, ZHANG Hui, WANG Bao-Shan   


For salinity stress tolerance in plants, the vacuolar type H+-ATPase (V-H+-ATPase) is of prime importance in establishing an electrochemical H+-gradient across tonoplast that energizes sodium sequestration into the central vacuole. In this paper, the sequence of a cDNA encoding the B subunit of the vacuolar-type H+-ATPase from Suaeda salsa L., a plant that can survive in seawater was reported. The B subunit cDNA is 1 974 nucleotides long and include a 18 bp poly(A+) tail together with a complete 1 470 bp coding region for a 489 amino acid with a conservative ATP binding site and a predicted molecular mass of 54.29 kD. Northern and Western blotting analyses indicated that the expression of B subunit was significantly up-regulated by NaCl treatment. Moreover, the expressions of B subunit were coordinated with c subunit of V-H+-ATPase at transcript and translation levels under NaCl stress. The increased V-H+-ATPase subunit amounts and activity of Suaeda salsa provide the energy for the compartmentation of sodium in response to salinity.

Key words: B subunit, c subunit, NaCl stress, Suaeda salsa , V-H+-ATPase

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q