J Integr Plant Biol. ›› 1994, Vol. 36 ›› Issue (Suppl.): -.

• Research Articles •    

Isolation and Purification of Megasporocarp and Microsporangium in Azolla

Zhang Gui-jin and Li Zhuo-shen   

  • Published:1994-12-31

Abstract: A simple and efficient method was developed to isolate and purify megasporocarp and microsporangium from Azolla. After dry powder of sporocarp-yielded A. microphyUa was imbibed for 48 hours, filterations were conducted sequently through three nets of 40-mesh (Φ 0.45 mm). 60-mesh (Φ 0.30 mm) and 80-mesh (Φ 0. 18 mm). so that magaporocarp or microsporangium were remained in the interception mixture on 60-mesh or 80-mesh net. Then the megasporocarp or microsporangium preparation Was spun in density gradient tube with 45% and 12% sucrose and water at 1000 r/min for 4 minutes in wing rotor, respectively. Finally. the megasporocarp floating in the upper layer of 12% sucrose or the microsporangium band between 45% and 12% sucrose layer were collected. With this method. 800±120 pure megasporocarps of 12000± 1200 pure microsporangia were harvested from 15 g fresh Azolla.

Key words: Azolla microphylls, Megasporocarp, microsporangium, Sucrose density gradient cetrifugation

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