J Integr Plant Biol. ›› 1994, Vol. 36 ›› Issue (4): -.

• Research Articles •    

Characterization of Nitrogen Fixation in Maize Root Associated Klebsiella planticola 19-1

Li Jiu-di, Wang Ji-wen, Li Yong-xing, Dong Yue-mei and Jin Hong-fan   

Abstract: A strain K19-1 was isolated from maize roots grown in sand of Xinjing Autonomous region. It was identified as Klebsiella planticola. K19-1 could grow on N2-free medium at 40℃ and reduce acetylene and 15N2 at 40℃. The nifH promoter: lacZ fusion was expressed in K19-1 at 40℃. NH4+ did not inhibit Nase activity but repressed the expression of nifH-lacZ fusion in K 19-1. K 19-1 could fix nitrogen anaerobically but Nase activity was still not inhibited till 02 concentration was increased to 4 %. The expression of nifH: lacZ fusion in K 19-1 was not repressed by O2.

Key words: Klebsiella planticola, Nase activity

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