J Integr Plant Biol. ›› 1993, Vol. 35 ›› Issue (Suppl.): -.

• Research Articles •    

Study on Production of Artificial Seeds of Hybrid Papaya

Ye Ke-nan,Huang Jun-chao and Li Bao-jian   

Abstract: Abundant embryogenic calli were induced from young roots and hypocotyls of hybrid papaya (Carica papaya L. ) on modified MS medium. After the calli were subcultured in liquid medium, a large number of somatic embryos were obtained. The different classes of cells were separated by screening the suspension through meshes of different sizes, a filtration step which served to synchronize somatic embryo development, thus, about 70% synchronous mature embryos were obtained. After desiccated to 50%--70% of water, dry somatic embryos were encapsulated in modified sodium alginate system which was composed of a mixture of sodium alginate with cellulose derivative A. The conversion rate of artificial seeds reached 80% under sterile condition and the survival rate of seedlings reached 72 % after they were transplanted to the field where the plants blossomed and yielded fruits normally.

Key words: Artificial seed, Desiccation and storage, Embryogenesis, Papaya

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q