J Integr Plant Biol. ›› 1993, Vol. 35 ›› Issue (Suppl.): -.

• Research Articles •    

Study on High-Frequeney Plant Regeneration from Monocell Suspension ,Cultures of Astragalus grubovii

An Li-jia, Zhang Jun-min,Li Feng-xia,Luo Xi-ming,He Meng-yuan and Hao Shui   

Abstract: Callus colony PL1 possessing the potential for a high frequency of plant regeneration (997.1%) was obtained from selected brown and green calli of Astragalus grubovii explants in subcultures. A relatively homogeneous cell suspension line was established from the callus colony in liquid Bx medium containing NO3- and NH4+ at a NO3-/NH4+ of 10:1 on a rotary shaker. Calli having a high frequency of plant regeneration (968.9%) were obtained from the the cell suspension line in monocell cultures.

Key words: Astragalus grubovii, Monocell, High-frequency, Plant regeneration

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